Author: Elste, James; Kaltenbach, Dominik; Patel, Vraj R.; Nguyen, Max T.; Sharthiya, Harsh; Tandon, Ritesh; Mehta, Satish K.; Volin, Michael V.; Fornaro, Michele; Tiwari, Vaibhav; Desai, Umesh R.
Title: Inhibition of Human Cytomegalovirus Entry into Host Cells through A Pleiotropic Small Molecule Document date: 2020_2_29
ID: 031ro01b_24
Snippet: The effect observed on the expression of important viral genes led to the prediction that SPGG possibly does not just function as a heparan sulfate competitor. We reasoned that SPGG may possibly bind to proteins involved in viral spread too. To test this hypothesis at a morphological level, rather than at a molecular level, we studied the phenomenon of viral spread using a plaque reduction assay. In this assay, we used β-galactosidase-expressing.....
Document: The effect observed on the expression of important viral genes led to the prediction that SPGG possibly does not just function as a heparan sulfate competitor. We reasoned that SPGG may possibly bind to proteins involved in viral spread too. To test this hypothesis at a morphological level, rather than at a molecular level, we studied the phenomenon of viral spread using a plaque reduction assay. In this assay, we used β-galactosidase-expressing reporter HCMV strain (RC256 from ATCC), which upon expression of β-galactosidase and x-gal staining in the infected cells showed blue foci. Plaque reduction assay have been considered the gold standard for antiviral susceptibility testing [68] .
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