Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018 Document date: 2019_11_6
ID: 0imlae98_3
Snippet: Most of the published research on RSV was performed using the laboratory strains RSV-Long and RSV A2, which were isolated from the population in 1956 and 1961, respectively [30, 31] . Not only have these viruses not circulated in the public for many years, they have been serially passaged in cell culture, which may have resulted in an accumulation of mutations that benefit the virus to grow in cell culture but may also affect virus-host interacti.....
Document: Most of the published research on RSV was performed using the laboratory strains RSV-Long and RSV A2, which were isolated from the population in 1956 and 1961, respectively [30, 31] . Not only have these viruses not circulated in the public for many years, they have been serially passaged in cell culture, which may have resulted in an accumulation of mutations that benefit the virus to grow in cell culture but may also affect virus-host interactions. Low-passage, characterized clinical strains are hard to come by and consequently less used in research. Therefore, we have isolated virus from mucosal secretions of 12 patients in the winter seasons of 2016-2017 and 2017-2018 in Belgium, resulting in eight RSV-A subtypes and four RSV-B subtypes. We have grown these viruses to passage 3 and used them to assess their viral replication kinetics and infectious virus production in HEp-2, A549 and BEAS-2B cells, thermal stability at 37 • C, 32 • C and 4 • C, syncytium formation and neutralization by palivizumab. We have also obtained G protein sequences to assign genotypes and evaluated production of mucin mRNA expression in A549 cells upon infection.
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