Selected article for: "assay kit and post infection"
Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate
  • Document date: 2018_5_16
    • ID: 1ghbutov_12
    Snippet: The human T-cell line MT4 cells were infected with HIV-1 NL4-3 , HIV-1 HXB2 or HIV-1 BH10 (1000 TCID 50 /mL) in the presence of the indicated concentration of PT-1 or positive control zidovudine (AZT). Two-hour post-infection, cells were washed with phosphate-buffered saline (PBS) and transferred to a 96-well plate. The cultures were incubated with medium replacement at day 3 and analyzed at day 5. The culture supernatants were harvested and quan.....
    Document: The human T-cell line MT4 cells were infected with HIV-1 NL4-3 , HIV-1 HXB2 or HIV-1 BH10 (1000 TCID 50 /mL) in the presence of the indicated concentration of PT-1 or positive control zidovudine (AZT). Two-hour post-infection, cells were washed with phosphate-buffered saline (PBS) and transferred to a 96-well plate. The cultures were incubated with medium replacement at day 3 and analyzed at day 5. The culture supernatants were harvested and quantified using a p24 enzyme-linked immunosorbent assay (ELISA) (Vironostika HIV-1 antigen ELISA kit, Biomerieux, Lyon, France).

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