Author: Singh, Manmeet; Khan, Reas S.; Dine, Kimberly; Das Sarma, Jayasri; Shindler, Kenneth S.
Title: Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis Document date: 2018_9_4
ID: 03c9rx3o_11
Snippet: RSA59 and RSMHV2, the isogenic recombinant strain of MHV-A59 and MHV2, respectively, were used as previously described (Das Sarma et al., 2009) . RSA59 and RSMHV2 are each engineered to express enhanced green fluorescence protein (EGFP), thus allowing viral antigen detection by fluorescence without immunohistochemical staining (Das Sarma et al., 2002) . Mice were monitored daily for signs of MHV induced neurologic up to 26 days (chronic stage) po.....
Document: RSA59 and RSMHV2, the isogenic recombinant strain of MHV-A59 and MHV2, respectively, were used as previously described (Das Sarma et al., 2009) . RSA59 and RSMHV2 are each engineered to express enhanced green fluorescence protein (EGFP), thus allowing viral antigen detection by fluorescence without immunohistochemical staining (Das Sarma et al., 2002) . Mice were monitored daily for signs of MHV induced neurologic up to 26 days (chronic stage) post-infection.
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