Author: Singh, Manmeet; Khan, Reas S.; Dine, Kimberly; Das Sarma, Jayasri; Shindler, Kenneth S.
Title: Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis Document date: 2018_9_4
ID: 03c9rx3o_13
Snippet: 50% LD50 doses of strains RSA59 (70,000 PFU), and RSMHV2 (1000 PFU) were used to inoculate 4-week-old, MHV-free, C57BL/6J mice (Jackson Laboratory). Desired PFU of viruses were prepared in a total volume of 20 µl in PBS and were pipeted as intranasal drops noninvasively every 2 min to alternating nares until all 20 µl were delivered, with simultaneous occlusion of the opposite naris. Drops were placed at the opening of the nares, allowing them .....
Document: 50% LD50 doses of strains RSA59 (70,000 PFU), and RSMHV2 (1000 PFU) were used to inoculate 4-week-old, MHV-free, C57BL/6J mice (Jackson Laboratory). Desired PFU of viruses were prepared in a total volume of 20 µl in PBS and were pipeted as intranasal drops noninvasively every 2 min to alternating nares until all 20 µl were delivered, with simultaneous occlusion of the opposite naris. Drops were placed at the opening of the nares, allowing them to be snorted into the nasal cavity. The mice for day 26 studies were also inoculated intracranially as a positive control for disease pathogenesis with RSA59, as in prior studies (Das Sarma et al., 2009) . Control mice mock-infected with PBS were inoculated in parallel. Animals were euthanized (3 mice per group) at day 1 post-inoculation (p.i.) and day 26 p.i.
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