Author: Connon, Richard E; Geist, Juergen; Pfeiff, Janice; Loguinov, Alexander V; D'Abronzo, Leandro S; Wintz, Henri; Vulpe, Christopher D; Werner, Inge
Title: Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae) Document date: 2009_12_15
ID: 1ecqnstz_53
Snippet: RNA was extracted from whole, individual organisms using a standard phenol:chloroform protocol with Trizol Reagent (Invitrogen). Fifteen micrograms of total RNA were used for cDNA synthesis, spiked with control RNA (CAB, RCA, RBCL and LTP4 (SpotReport, Stratagene) and labeled with Alexa fluor dyes, using SuperScriptâ„¢ Plus Indirect cDNA labeling System (Invitrogen). Each experimental sample and control was combined with a reference pool cDNA pri.....
Document: RNA was extracted from whole, individual organisms using a standard phenol:chloroform protocol with Trizol Reagent (Invitrogen). Fifteen micrograms of total RNA were used for cDNA synthesis, spiked with control RNA (CAB, RCA, RBCL and LTP4 (SpotReport, Stratagene) and labeled with Alexa fluor dyes, using SuperScriptâ„¢ Plus Indirect cDNA labeling System (Invitrogen). Each experimental sample and control was combined with a reference pool cDNA prior to hybridization using an automated Tecan HS4800 hybridization station. Slides were scanned using a GenePix 4000B scanner (Axon Instruments).
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