Selected article for: "end point and GP rvsv"
Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection
  • Document date: 2015_11_12
    • ID: 10bu7iwg_52
    Snippet: EBOV GP/rVSV. Cytokine-containing media was removed from the macrophages and replaced with RPMI 1460 and 10% FCS. For the end point dilution assays, human and murine macrophage populations were infected with 10-fold serial dilutions of EBOV GP/rVSV (at least 4 replicates per dilution). Infection was scored 24 hours following infection for GFP positivity using a fluorescent microscope. Virus titers were calculated as the 50% tissue culture infecti.....
    Document: EBOV GP/rVSV. Cytokine-containing media was removed from the macrophages and replaced with RPMI 1460 and 10% FCS. For the end point dilution assays, human and murine macrophage populations were infected with 10-fold serial dilutions of EBOV GP/rVSV (at least 4 replicates per dilution). Infection was scored 24 hours following infection for GFP positivity using a fluorescent microscope. Virus titers were calculated as the 50% tissue culture infective dose (TCID50)/mL by the Reed-Meunch method [90] .

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