Author: Suddala, Krishna C.; Lee, Christine C.; Meraner, Paul; Marin, Mariana; Markosyan, Ruben M.; Desai, Tanay M.; Cohen, Fredric S.; Brass, Abraham L.; Melikyan, Gregory B.
Title: Interferon-induced transmembrane protein 3 blocks fusion of sensitive but not resistant viruses by partitioning into virus-carrying endosomes Document date: 2019_1_14
ID: 15wxk8lt_20
Snippet: Further analysis of time-lapse acquisitions performed in at least 15 independent experiments shows that, on average, 2.2% of IAVpp fuse in Vector cells and none of the 6309 analyzed particles in IFITM3+ endosomes underwent fusion ( Fig 3J) . Our results thus demonstrate, for the first time, that the presence of IFITM3 in the endosomes carrying the virus is key to restriction of IAV fusion. The unimpeded lipid mixing between IAV and IFITM3+ endoso.....
Document: Further analysis of time-lapse acquisitions performed in at least 15 independent experiments shows that, on average, 2.2% of IAVpp fuse in Vector cells and none of the 6309 analyzed particles in IFITM3+ endosomes underwent fusion ( Fig 3J) . Our results thus demonstrate, for the first time, that the presence of IFITM3 in the endosomes carrying the virus is key to restriction of IAV fusion. The unimpeded lipid mixing between IAV and IFITM3+ endosomes, together with the lack of viral content release, strongly imply that IFITM3 traps the IAV fusion at a hemifusion stage by blocking the formation of a small fusion pore (in agreement with our previous study [30] ).
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