Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018 Document date: 2019_11_6
ID: 0imlae98_17
Snippet: Aliquots of clinical isolates and RSV A2 and RSV B1 were thawed, diluted in DMEM-0 to obtain a starting concentration of 1 × 10 5 PFU/mL and aliquots were made of each sample. Immediately afterwards, one aliquot of each sample was snap frozen in liquid nitrogen as T0. The other aliquots were stored at 4 • C, at 32 • C or at 37 • C for 24 h, 48 h and 72 h, snap frozen in liquid nitrogen and stored at -80 • C until quantification was perfo.....
Document: Aliquots of clinical isolates and RSV A2 and RSV B1 were thawed, diluted in DMEM-0 to obtain a starting concentration of 1 × 10 5 PFU/mL and aliquots were made of each sample. Immediately afterwards, one aliquot of each sample was snap frozen in liquid nitrogen as T0. The other aliquots were stored at 4 • C, at 32 • C or at 37 • C for 24 h, 48 h and 72 h, snap frozen in liquid nitrogen and stored at -80 • C until quantification was performed. A conventional plaque assay on HEp-2 cells, as described above, was used to quantify the remaining PFU in each aliquot.
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