Author: Lee, Yu-Ching; Tsai, Keng-Chang; Leu, Sy-Jye; Wang, Tuan-Jen; Liu, Chia-Yu; Yang, Yi-Yuan
Title: Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren's Syndrome Document date: 2013_12_30
ID: 0zsn4lu3_17
Snippet: 2.6. DNA Sequence Analysis. Light and heavy chain inserts of Fc-binding clones were sequenced and analyzed. Primers complementary to constant region of heavy chain (SeqGb: 5 -GTC GTT GAC CAG GCA GCC CAG-3 (21 mers)) and lambda light chain (SeqLb: 5 -GAA GTC ACT TAT GAG ACA CAC-3 (21 mers)) were used. The IMGT/V-QUEST international immunogenetics information system (http://www.imgt.org/)was used to compile and analyze sequence data in parallel wit.....
Document: 2.6. DNA Sequence Analysis. Light and heavy chain inserts of Fc-binding clones were sequenced and analyzed. Primers complementary to constant region of heavy chain (SeqGb: 5 -GTC GTT GAC CAG GCA GCC CAG-3 (21 mers)) and lambda light chain (SeqLb: 5 -GAA GTC ACT TAT GAG ACA CAC-3 (21 mers)) were used. The IMGT/V-QUEST international immunogenetics information system (http://www.imgt.org/)was used to compile and analyze sequence data in parallel with germline gene.
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