Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_15
Snippet: HEK-293 cells were infected with 360 plaque forming units (PFU) of CHPV. Cells were transfected with 100 pmol of different siRNAs at 2 hr PI. Cell supernatants were harvested 24 hr post transfection, RNA isolated using QIAmp viral RNA minikit (QIAGEN, Valencia, CA) and real time one step RT-PCR was carried out as described earlier [24] ......
Document: HEK-293 cells were infected with 360 plaque forming units (PFU) of CHPV. Cells were transfected with 100 pmol of different siRNAs at 2 hr PI. Cell supernatants were harvested 24 hr post transfection, RNA isolated using QIAmp viral RNA minikit (QIAGEN, Valencia, CA) and real time one step RT-PCR was carried out as described earlier [24] .
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