Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_36
Snippet: M gene siRNAs (M1-M8) were tested with the virus and efficiencies were compared with P-2 siRNA. The results showed P-2, M-5 and M-6 to be equally potent in inhibiting CHPV induced cytopathic effects as observed by phase-contrast microscopy (10X magnification) (Fig 3a) . Assessment of virus replication by plaque assay and real time one step RT-PCR showed inhibition of CHPV by 2logs and thus confirmed efficacy of all the three siRNAs (p,0.05) (Fig .....
Document: M gene siRNAs (M1-M8) were tested with the virus and efficiencies were compared with P-2 siRNA. The results showed P-2, M-5 and M-6 to be equally potent in inhibiting CHPV induced cytopathic effects as observed by phase-contrast microscopy (10X magnification) (Fig 3a) . Assessment of virus replication by plaque assay and real time one step RT-PCR showed inhibition of CHPV by 2logs and thus confirmed efficacy of all the three siRNAs (p,0.05) (Fig 3b) .
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