Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018 Document date: 2019_11_6
ID: 0imlae98_39
Snippet: Differences in the F protein are shown to be involved in thermal stability of viral particles [37] . Aliquots of each virus containing 1×10 5 PFU/mL were incubated at three different temperatures: 37 °C (in vitro incubator temperature and core body temperature) ( Figure 5A,B) , 32 °C (upper airway Overall, clinical isolate BE/ANT-A11/17 replicated very efficiently in all cell lines, and remarkably, achieving even higher infection rates in the .....
Document: Differences in the F protein are shown to be involved in thermal stability of viral particles [37] . Aliquots of each virus containing 1×10 5 PFU/mL were incubated at three different temperatures: 37 °C (in vitro incubator temperature and core body temperature) ( Figure 5A,B) , 32 °C (upper airway Overall, clinical isolate BE/ANT-A11/17 replicated very efficiently in all cell lines, and remarkably, achieving even higher infection rates in the HEp-2 cell line than the RSV A2. Additionally, two clinical isolates of the RSV-B subtype (BE/ANT-B20/17 and BE/ANT-B13/17) replicated very well in HEp-2 and A549 cell lines and quite well in BEAS-2B. Overall, differences in infection kinetics were observed also among the different clinical isolates.
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