Selected article for: "Germany Qiagen RNA mini kit and viral Germany Qiagen RNA mini kit"
Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018
  • Document date: 2019_11_6
    • ID: 0imlae98_9
    Snippet: RNA for subtyping was extracted from passage 0 virus using the QIamp viral RNA extraction mini kit (QIAgen, Hilden, Germany) following the instructions of the manufacturer. A multiplex reaction mix was made with superscript III platinum one-step quantitative kit (Thermo Fisher Scientific) in a final volume of 25 µL containing 5 µL RNA, 12.5µL PCR master mix, 1 µL superscript RT/Platinum Taq polymerase and 2.5 µL of a pre-mixed primer/probe s.....
    Document: RNA for subtyping was extracted from passage 0 virus using the QIamp viral RNA extraction mini kit (QIAgen, Hilden, Germany) following the instructions of the manufacturer. A multiplex reaction mix was made with superscript III platinum one-step quantitative kit (Thermo Fisher Scientific) in a final volume of 25 µL containing 5 µL RNA, 12.5µL PCR master mix, 1 µL superscript RT/Platinum Taq polymerase and 2.5 µL of a pre-mixed primer/probe solution. This solution contains a final concentration of 5 µM of each primer and 1 µM of each probe. The primers for RSV-A are located in the

    Search related documents:
    Co phrase search for related documents
    • extraction mini kit and RNA Hilden QIAgen extraction mini kit: 1, 2
    • final concentration and reaction mix: 1, 2, 3, 4, 5, 6
    • final concentration and RT superscript: 1, 2
    • final volume and reaction mix: 1, 2, 3, 4
    • final volume and RT superscript: 1, 2
    • Hilden QIAgen extraction mini kit and RNA Hilden QIAgen extraction mini kit: 1, 2
    • III platinum and reaction mix: 1, 2
    • III platinum and RT superscript: 1, 2, 3, 4, 5, 6, 7, 8
    • master mix and quantitative kit: 1
    • master mix and reaction mix: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • master mix and RSV primer: 1, 2
    • master mix and RT superscript: 1, 2, 3
    • mini kit and quantitative kit: 1, 2
    • mini kit and reaction mix: 1, 2, 3, 4
    • mini kit and RNA Hilden QIAgen extraction mini kit: 1, 2, 3
    • mini kit and RT superscript: 1, 2, 3, 4
    • multiplex reaction mix and reaction mix: 1, 2, 3
    • PCR master mix and quantitative kit: 1
    • PCR master mix and reaction mix: 1, 2, 3, 4, 5, 6, 7, 8