Selected article for: "final volume and mini kit"
Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018
  • Document date: 2019_11_6
    • ID: 0imlae98_9
    Snippet: RNA for subtyping was extracted from passage 0 virus using the QIamp viral RNA extraction mini kit (QIAgen, Hilden, Germany) following the instructions of the manufacturer. A multiplex reaction mix was made with superscript III platinum one-step quantitative kit (Thermo Fisher Scientific) in a final volume of 25 µL containing 5 µL RNA, 12.5µL PCR master mix, 1 µL superscript RT/Platinum Taq polymerase and 2.5 µL of a pre-mixed primer/probe s.....
    Document: RNA for subtyping was extracted from passage 0 virus using the QIamp viral RNA extraction mini kit (QIAgen, Hilden, Germany) following the instructions of the manufacturer. A multiplex reaction mix was made with superscript III platinum one-step quantitative kit (Thermo Fisher Scientific) in a final volume of 25 µL containing 5 µL RNA, 12.5µL PCR master mix, 1 µL superscript RT/Platinum Taq polymerase and 2.5 µL of a pre-mixed primer/probe solution. This solution contains a final concentration of 5 µM of each primer and 1 µM of each probe. The primers for RSV-A are located in the

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