Author: Yu, Liping; Zhang, Xiaorong; Wu, Tianqi; Su, Jin; Wang, Yuyang; Wang, Yuexin; Ruan, Baoyang; Niu, Xiaosai; Wu, Yantao
Title: Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVS Document date: 2017_11_13
ID: 0zn1sqj9_13
Snippet: Eight housekeeping were screened to identify the most stably expressed reference genes in different chicken embryo tissues: β-actin (ACTB) [27] , testis-specific alpha-tubulin mRNA (TUBAT) [28] , Mitochondrial ribosomal protein S30 (MRPS30) [29] , Eukaryotic translation elongation factor 1 alpha 2 (EFF1) [29] , Guanine nucleotide binding protein (G protein), ribosomal protein L32 [30] , β-glucuronidase (GUSB) [31] , glyceraldehyde-3-phosphate d.....
Document: Eight housekeeping were screened to identify the most stably expressed reference genes in different chicken embryo tissues: β-actin (ACTB) [27] , testis-specific alpha-tubulin mRNA (TUBAT) [28] , Mitochondrial ribosomal protein S30 (MRPS30) [29] , Eukaryotic translation elongation factor 1 alpha 2 (EFF1) [29] , Guanine nucleotide binding protein (G protein), ribosomal protein L32 [30] , β-glucuronidase (GUSB) [31] , glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [31] and Ribosomal protein L5 (RPL5) [29] . To calculate the stability of reference genes, three different analysis methods (geNorm, NormFinder and Best-Keeper) were used [30] [31] [32] . (Additional file 1: Methods and Table S1 ).
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