Author: Lee, Yu-Ching; Tsai, Keng-Chang; Leu, Sy-Jye; Wang, Tuan-Jen; Liu, Chia-Yu; Yang, Yi-Yuan
Title: Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren's Syndrome Document date: 2013_12_30
ID: 0zsn4lu3_13
Snippet: To examine the Fab expression, the IPTG-induced bacterial lysates were subjected to sodium dodecyl sulfate polyacrylamide gel for electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membrane (GE Biosciences, UK), followed by blocking with 5% skim milk in PBST for 1 hr at room temperature with gently shaking. After washing, HRP-conjugated goat anti-human or light chain antibodies were added and incubated for one additional hour at room .....
Document: To examine the Fab expression, the IPTG-induced bacterial lysates were subjected to sodium dodecyl sulfate polyacrylamide gel for electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membrane (GE Biosciences, UK), followed by blocking with 5% skim milk in PBST for 1 hr at room temperature with gently shaking. After washing, HRP-conjugated goat anti-human or light chain antibodies were added and incubated for one additional hour at room temperature with gently shaking. After washing with PBST, the membranes were developed with diaminobenzidine (DAB) substrate until desired intensity was reached.
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