Selected article for: "II kit and Nucleospin Macherey Nagel RNA II kit"
Author: Barker, Emily N.; Stranieri, Angelica; Helps, Chris R.; Porter, Emily L.; Davidson, Andrew D.; Day, Michael J.; Knowles, Toby; Kipar, Anja; Tasker, Séverine
Title: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis
  • Document date: 2017_10_5
    • ID: 08b0g46x_14
    Snippet: Total RNA was extracted from 20 mg of tissue with a NucleoSpin RNA II kit (Macherey-Nagel) using methods previously described [23] . Total RNA was extracted from 100 µL body cavity fluid or 10 mg of faeces or faecal swab using either the NucleoSpin RNA II kit or using an automated platform Chemagic 360 instrument (Perkin-Elmer) in combination with Chemagic body fluids NA kit (Perkin-Elmer) eluted in 100 µL elution buffer......
    Document: Total RNA was extracted from 20 mg of tissue with a NucleoSpin RNA II kit (Macherey-Nagel) using methods previously described [23] . Total RNA was extracted from 100 µL body cavity fluid or 10 mg of faeces or faecal swab using either the NucleoSpin RNA II kit or using an automated platform Chemagic 360 instrument (Perkin-Elmer) in combination with Chemagic body fluids NA kit (Perkin-Elmer) eluted in 100 µL elution buffer.

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