Selected article for: "additional file and target gene"
Author: Barker, Emily N.; Stranieri, Angelica; Helps, Chris R.; Porter, Emily L.; Davidson, Andrew D.; Day, Michael J.; Knowles, Toby; Kipar, Anja; Tasker, Séverine
Title: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis
  • Document date: 2017_10_5
    • ID: 08b0g46x_31
    Snippet: Of the 320 FCoV RT-qPCR-positive samples subjected to S gene mutation analysis 272 (85.0%) were successfully pyrosequenced (n = 89 at nucleotide 23531 alone, for M1058L; n = 183 at nucleotides 23531 and 23537, for M1058L and S1060A), 21 (6.6%) required Sanger sequencing, and 27 (8.4%) failed target gene sequencing. For the outcome of S gene analysis of FCoV RT-qPCRpositive samples see Table 3 (individual results can be found in Additional file 1).....
    Document: Of the 320 FCoV RT-qPCR-positive samples subjected to S gene mutation analysis 272 (85.0%) were successfully pyrosequenced (n = 89 at nucleotide 23531 alone, for M1058L; n = 183 at nucleotides 23531 and 23537, for M1058L and S1060A), 21 (6.6%) required Sanger sequencing, and 27 (8.4%) failed target gene sequencing. For the outcome of S gene analysis of FCoV RT-qPCRpositive samples see Table 3 (individual results can be found in Additional file 1).

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