Author: Barker, Emily N.; Stranieri, Angelica; Helps, Chris R.; Porter, Emily L.; Davidson, Andrew D.; Day, Michael J.; Knowles, Toby; Kipar, Anja; Tasker, Séverine
Title: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis Document date: 2017_10_5
ID: 08b0g46x_48
Snippet: Four fluid samples had non-mutated FCoVs detected; these comprised abdominal fluid (n = 3) and pleural fluid (n = 1) from three cats (#55, #70 and #93); two of which had mutated FCoVs in tissue samples (#55 and #93), and one of which had both non-mutated and mixed mutated FCoVs in tissue samples (#70). Four samples had sequence data consistent with the presence of both nonmutated and mutated FCoVs; these fluids from three cats (#37, #79 and #103).....
Document: Four fluid samples had non-mutated FCoVs detected; these comprised abdominal fluid (n = 3) and pleural fluid (n = 1) from three cats (#55, #70 and #93); two of which had mutated FCoVs in tissue samples (#55 and #93), and one of which had both non-mutated and mixed mutated FCoVs in tissue samples (#70). Four samples had sequence data consistent with the presence of both nonmutated and mutated FCoVs; these fluids from three cats (#37, #79 and #103) were of abdominal (n = 1), pleural (n = 2), and unknown origin (n = 1); all cats had mutated FCoVs in tissue samples. Mutated FCoVs were detected in 26 samples; 25 with the M1058L mutation (nucleotide 23531A>T n = 21; nucleotide 23531A>C n = 4); one with the S1060A mutation.
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