Author: Connon, Richard E; Geist, Juergen; Pfeiff, Janice; Loguinov, Alexander V; D'Abronzo, Leandro S; Wintz, Henri; Vulpe, Christopher D; Werner, Inge
Title: Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae) Document date: 2009_12_15
ID: 1ecqnstz_66
Snippet: Quantitative PCR data was analyzed using the relative quantification 2(-Delta Delta CT) method ()([93]. In the absence of house keeping genes, expression was calculated relative to the mean of controls in respective exposures. Surviving larvae from each replicate of the 10-d old exposed delta smelt were used for q-PCR analyses. Oneway ANOVA was used to assess differences in gene expression through out the exposure concentrations, and data were fu.....
Document: Quantitative PCR data was analyzed using the relative quantification 2(-Delta Delta CT) method ()([93]. In the absence of house keeping genes, expression was calculated relative to the mean of controls in respective exposures. Surviving larvae from each replicate of the 10-d old exposed delta smelt were used for q-PCR analyses. Oneway ANOVA was used to assess differences in gene expression through out the exposure concentrations, and data were further assessed using Student's T-test at individual concentrations in respect to solvent controls.
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