Selected article for: "cancer cell and expression level"
Author: Li, Lv; Dai, Hong-Juan; Ye, Mao; Wang, Shu-Ling; Xiao, Xiao-Juan; Zheng, Jie; Chen, Hui-Yong; Luo, Yu-hao; Liu, Jing
Title: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition
  • Document date: 2012_11_23
    • ID: 13amcxh2_12
    Snippet: To examine the effect of lycorine on the phosphorylation of pRB, K562 cells were treated with different concentrations of lycorine, after which proteins were detected using antibodies specific to the total pRB and phosphorylated pRB. Results show that the expression of total pRB remains almost unchanged but the level of phosphorylated pRB decreases significantly in a dosedependent manner ( Figure 5) . p21, as a CDK inhibitor, can interfere with c.....
    Document: To examine the effect of lycorine on the phosphorylation of pRB, K562 cells were treated with different concentrations of lycorine, after which proteins were detected using antibodies specific to the total pRB and phosphorylated pRB. Results show that the expression of total pRB remains almost unchanged but the level of phosphorylated pRB decreases significantly in a dosedependent manner ( Figure 5) . p21, as a CDK inhibitor, can interfere with cancer cell cycle and affect cell proliferation [12] . p21 binds to and inhibits the activity of cyclin E-CDK2 complexes, which cause pRB hypophosphorylation and cell-cycle arrest at the G1-S transition. We further explored the expression of p21 at the protein level and found that lycorine could induce a dose-dependent increase in p21 in K562 cells ( Figure 5 ). Consistent with the change in p21, the expression of p53 protein was also elevated, which suggests that lycorine induces the expression of p21 in a p53-dependent manner in K562 cells ( Figure 5 ).

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