Selected article for: "bovine serum and humidified atmosphere"
Author: Li, Lv; Dai, Hong-Juan; Ye, Mao; Wang, Shu-Ling; Xiao, Xiao-Juan; Zheng, Jie; Chen, Hui-Yong; Luo, Yu-hao; Liu, Jing
Title: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition
  • Document date: 2012_11_23
    • ID: 13amcxh2_21
    Snippet: The human CML cell line K562 was purchased from American Type Culture Collection and cultivated in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 100 U/mL streptomycin, and 100 U/mL penicillin at 37°C in a humidified atmosphere with 5% CO 2 . Cells were diluted at a ratio of 1:3 every 1 d to 2 d. Lycorine (Sigma) was dissolved at 0.034 M in dimethyl sulfoxide (DMSO; Sigma) as a stock solution and dilu.....
    Document: The human CML cell line K562 was purchased from American Type Culture Collection and cultivated in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 100 U/mL streptomycin, and 100 U/mL penicillin at 37°C in a humidified atmosphere with 5% CO 2 . Cells were diluted at a ratio of 1:3 every 1 d to 2 d. Lycorine (Sigma) was dissolved at 0.034 M in dimethyl sulfoxide (DMSO; Sigma) as a stock solution and diluted in serum-free RPMI 1640 medium just before use. The maximum final concentration of DMSO in medium was less than 0.02%.

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