Author: Yang, Liu; Du, Xing; Liu, Lu; Cao, Qiuyu; Pan, Zengxiang; Li, Qifa
Title: miR-1306 Mediates the Feedback Regulation of the TGF-ß/SMAD Signaling Pathway in Granulosa Cells Document date: 2019_3_31
ID: 16qix4ab_6
Snippet: Porcine TGF-β1 was obtained from R&D Systems (Minneapolis, MN, USA). SMAD4-siRNA, TGFBR2-siRNA, miR-1306 mimics, miR-1306 inhibitors, and their negative control (NC) (NC-siRNA, mimic NC and inhibitor NC) were synthesized and purified by GenePharma (Shanghai, China) (Supplementary Table S1 ). Antibodies against phospho-SMAD3 (p-SMAD3) (Catalog #D155153-0025), TGFBR2 (Catalog #D151818-0025), SMAD3 (Catalog #D155234-0100), glyceraldehyde-3-phosphat.....
Document: Porcine TGF-β1 was obtained from R&D Systems (Minneapolis, MN, USA). SMAD4-siRNA, TGFBR2-siRNA, miR-1306 mimics, miR-1306 inhibitors, and their negative control (NC) (NC-siRNA, mimic NC and inhibitor NC) were synthesized and purified by GenePharma (Shanghai, China) (Supplementary Table S1 ). Antibodies against phospho-SMAD3 (p-SMAD3) (Catalog #D155153-0025), TGFBR2 (Catalog #D151818-0025), SMAD3 (Catalog #D155234-0100), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Catalog #D198662-0100), horseradish peroxidase (HRP)-conjugated mouse anti-rabbit (Catalog #D110065-0100), and HRP-conjugated goat anti-mouse immunoglobulin G (IgG) (Catalog # 110087-0100) were purchased from Sangon Biotech (Shanghai, China). Dulbecco's minimum essential medium/nutrient F-12 (DMEM/F-12), fetal bovine serum (FBS), and Opti-MEM were purchased from Gibco (USA). Phosphate-buffered saline (PBS) was obtained from HyClone (USA). Lipofectamine 2000 was obtained from Invitrogen (Carlsbad, CA, USA). The protease and phosphatase inhibitors were purchased from Roche (Basel, Switzerland).
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