Author: Shabman, Reed S.; Shrivastava, Susmita; Tsibane, Tshidi; Attie, Oliver; Jayaprakash, Anitha; Mire, Chad E.; Dilley, Kari E.; Puri, Vinita; Stockwell, Timothy B.; Geisbert, Thomas W.; Sachidanandam, Ravi; Basler, Christopher F.
Title: Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line Document date: 2016_2_17
ID: 1a9u53za_9
Snippet: To prove that infectivity of the BGHV8 can be propagated beyond a single passage in cell culture, we passaged the virus multiple times in Vero cells (data not shown). Since the virus was able to be efficiently propagated, we optimized assays to measure infectivity, including a plaque assay (Fig. 2C ) and a 50% tissue culture infectious dose (TCID 50 ) assay ( Fig. 2D ) on Vero cells, the most permissive cell line tested. BGHV8 completely destroys.....
Document: To prove that infectivity of the BGHV8 can be propagated beyond a single passage in cell culture, we passaged the virus multiple times in Vero cells (data not shown). Since the virus was able to be efficiently propagated, we optimized assays to measure infectivity, including a plaque assay (Fig. 2C ) and a 50% tissue culture infectious dose (TCID 50 ) assay ( Fig. 2D ) on Vero cells, the most permissive cell line tested. BGHV8 completely destroys Vero monolayers, and the corresponding supernatants produce virus titers between 10 5 and 10 6 PFU/TCID 50 per milliliter of supernatant. Taken together, these data indicate that BGHV8 undergoes lytic replication in Vero cells, allowing infectious virus to be quantified.
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