Author: Zhang, Qingshui; Cao, Yanxin; Wang, Jun; Fu, Guanghua; Sun, Mengxu; Zhang, Lijiao; Meng, Li; Cui, Guolin; Huang, Yu; Hu, Xueying; Su, Jingliang
Title: Isolation and characterization of an astrovirus causing fatal visceral gout in domestic goslings Document date: 2018_4_19
ID: 1k5jucae_29
Snippet: To determine the infectious titers of the 4 th and 9 th passage, the virus suspension was 10-fold serially diluted with PBS and inoculated into 9-day-old goose embryos via the chorioallantoic membrane route. The embryos were incubated for 7 days at 37°C and the mean embryo lethal dose (ELD 50 ) of infectious virus was calculated using the Reed-Muench method 32 ......
Document: To determine the infectious titers of the 4 th and 9 th passage, the virus suspension was 10-fold serially diluted with PBS and inoculated into 9-day-old goose embryos via the chorioallantoic membrane route. The embryos were incubated for 7 days at 37°C and the mean embryo lethal dose (ELD 50 ) of infectious virus was calculated using the Reed-Muench method 32 .
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