Author: Barker, Emily N.; Stranieri, Angelica; Helps, Chris R.; Porter, Emily L.; Davidson, Andrew D.; Day, Michael J.; Knowles, Toby; Kipar, Anja; Tasker, Séverine
Title: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis Document date: 2017_10_5
ID: 08b0g46x_69
Snippet: When calculating accuracy, the authors considered it very important to include samples for which S gene mutation analysis failed, as a more accurate reflection of the clinical situation where failure to identify the presence of mutated FCoVs may be considered lack of support of the diagnosis of FIP. We were not able to obtain pyrosequencing data in all samples; this was attributed to low relative FCoV copy number, failure of amplification primer .....
Document: When calculating accuracy, the authors considered it very important to include samples for which S gene mutation analysis failed, as a more accurate reflection of the clinical situation where failure to identify the presence of mutated FCoVs may be considered lack of support of the diagnosis of FIP. We were not able to obtain pyrosequencing data in all samples; this was attributed to low relative FCoV copy number, failure of amplification primer binding, or failure of pyrosequencing primers to bind to viral cDNA as a result of sequence mismatches.
Search related documents:
Co phrase search for related documents- copy number and FIP diagnosis: 1, 2
- copy number and gene mutation: 1, 2, 3, 4, 5, 6, 7, 8
- copy number and gene mutation analysis: 1
- FCoV copy number and FIP diagnosis: 1, 2
- FCoV copy number and gene mutation: 1, 2
- FCoV copy number and gene mutation analysis: 1
- FIP diagnosis and gene mutation: 1, 2, 3, 4, 5, 6, 7
- FIP diagnosis and gene mutation analysis: 1, 2, 3, 4, 5
- FIP diagnosis and primer pyrosequence: 1
- gene mutation analysis and primer pyrosequence: 1
- gene mutation and primer pyrosequence: 1
Co phrase search for related documents, hyperlinks ordered by date