Author: Li, Lv; Dai, Hong-Juan; Ye, Mao; Wang, Shu-Ling; Xiao, Xiao-Juan; Zheng, Jie; Chen, Hui-Yong; Luo, Yu-hao; Liu, Jing
Title: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition Document date: 2012_11_23
ID: 13amcxh2_30
Snippet: Flow cytometry was used to detect the cell cycle distribution and quantitatively measure the apoptotic rate. After K562 cells treated with lycorine (5.0 μM) or without lycorine were cultivated at 5 × 10 5 cells/mL in each culture flask (BioCoat) for 24 h, 1 × 10 6 cells were harvested and washed with PBS. The cells were then fixed with ice-cold 70% ethanol at −20°C overnight. The next day, the cells were washed with PBS, stained with 50 mg/.....
Document: Flow cytometry was used to detect the cell cycle distribution and quantitatively measure the apoptotic rate. After K562 cells treated with lycorine (5.0 μM) or without lycorine were cultivated at 5 × 10 5 cells/mL in each culture flask (BioCoat) for 24 h, 1 × 10 6 cells were harvested and washed with PBS. The cells were then fixed with ice-cold 70% ethanol at −20°C overnight. The next day, the cells were washed with PBS, stained with 50 mg/mL propidium iodide (Sigma), and dissolved in 100 mg/L RNase A (Sigma). The sub-G1 peak (apoptosis percentage) and cell cycle distribution were measured with Cytomic FC 500 (Beckman Coulter) and analyzed using Modifit LT software.
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