Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae)

Connon, Richard E; Geist, Juergen; Pfeiff, Janice; Loguinov, Alexander V; D'Abronzo, Leandro S; Wintz, Henri; Vulpe, Christopher D; Werner, Inge

We constructed a delta smelt microarray using 8448 PCR amplified fragments from a normalized cDNA library. A cDNA library was created using expressed sequence tags (ESTs) ligated into p-BS plasmid vectors and cloned into chemically competent Escherichia coli cells (BioS&T Inc, Montreal, Quebec, Canada). RNA for library construction was obtained from a number of larval, juvenile and adult delta smelt, ranging from unexposed, control conditions, to fish from exposures to high temperature (25°C), and sublethal concentrations of copper, esfenvalerate, and a six field water samples from throughout the Sacramento-San Joaquin estuary. Products were PCR amplified from 1 μl bacterial suspension, and visualized on agarose gels.