Author: Burbelo, Peter D.; Chaturvedi, Adrija; Notkins, Abner L.; Gunti, Sreenivasulu
Title: Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma Document date: 2019_8_6
ID: 06glboqq_7
Snippet: Previously described pREN2 constructs for Renilla luciferase (Ruc) and p53 chimeric fusion protein were used [25] . Two known p53 cancer mutant proteins, p53-R175H and p53-R273H, were also generated in pREN2. The coding sequences for E2, E6 and E7 proteins from HPV-16 were subcloned into the pREN2 vector for generating C-terminal HPV fusion proteins essentially as described for other antigens [25] . The E6 cDNA was also subcloned into the pNano2 .....
Document: Previously described pREN2 constructs for Renilla luciferase (Ruc) and p53 chimeric fusion protein were used [25] . Two known p53 cancer mutant proteins, p53-R175H and p53-R273H, were also generated in pREN2. The coding sequences for E2, E6 and E7 proteins from HPV-16 were subcloned into the pREN2 vector for generating C-terminal HPV fusion proteins essentially as described for other antigens [25] . The E6 cDNA was also subcloned into the pNano2 vector for expression as a NanoLuc fusion protein [24] , a highly active luciferase. The integrity of the described constructs was confirmed by DNA sequencing.
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