Selected article for: "assay kit and Extraction kit"
Author: Li, Lv; Dai, Hong-Juan; Ye, Mao; Wang, Shu-Ling; Xiao, Xiao-Juan; Zheng, Jie; Chen, Hui-Yong; Luo, Yu-hao; Liu, Jing
Title: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition
  • Document date: 2012_11_23
    • ID: 13amcxh2_28
    Snippet: A HDAC colorimetric assay kit (Biovision) was applied to determine HDAC enzymatic activities in the cell nucleus according to the manufacturer's instructions. Briefly, proteins were extracted from K562 cells treated with different concentrations of lycorine (2.5 or 5.0 μM) or without lycorine for 24 h using a nuclear and cytoplasmic protein extraction kit (Beyotime) according to manufacturer recommendations. About 50 μg of nuclear protein from .....
    Document: A HDAC colorimetric assay kit (Biovision) was applied to determine HDAC enzymatic activities in the cell nucleus according to the manufacturer's instructions. Briefly, proteins were extracted from K562 cells treated with different concentrations of lycorine (2.5 or 5.0 μM) or without lycorine for 24 h using a nuclear and cytoplasmic protein extraction kit (Beyotime) according to manufacturer recommendations. About 50 μg of nuclear protein from each group was added to a 96-well tissue culture plate (BioCoat) at a final volume of 100 μL per well. After incubation, HDAC activities were measured by scanning with an ELISA reader with a 450 nm filter. Values were expressed as the percentage of HDAC activities relative to the untreated cell extract.

    Search related documents:
    Co phrase search for related documents
    • assay kit and colorimetric assay kit: 1, 2, 3, 4, 5, 6
    • assay kit and culture plate: 1, 2
    • assay kit and different concentration: 1
    • assay kit and ELISA reader: 1
    • assay kit and enzymatic activity: 1, 2, 3, 4, 5
    • assay kit and extraction kit: 1, 2, 3, 4, 5, 6, 7
    • assay kit and final volume: 1
    • BioCoat culture plate and culture plate: 1
    • BioCoat culture plate and ELISA reader: 1
    • biovision colorimetric assay kit and colorimetric assay kit: 1, 2
    • cell extract and final volume: 1
    • cell nucleus and enzymatic activity: 1
    • colorimetric assay kit and enzymatic activity: 1
    • culture plate and ELISA reader: 1, 2
    • culture plate and final volume: 1
    • different concentration and enzymatic activity: 1
    • different concentration and extraction kit: 1
    • different concentration and final volume: 1
    • ELISA reader and final volume: 1