Author: Allemandou, Aude; Grasland, Béatrice; Hernandez-Nignol, Anne-Cécile; Kéranflec'h, André; Cariolet, Roland; Jestin, André
Title: Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein Document date: 2011_3_24
ID: 1fl0we2a_15
Snippet: The sequence of each parental DNA clone was submitted as template for design primers on the QuickChange design program [23] . According to the manufacturer's instructions, mutagenesis was performed in two steps because of the primer length. For each mutant, two pairs of primers were designed ( Table 1) ......
Document: The sequence of each parental DNA clone was submitted as template for design primers on the QuickChange design program [23] . According to the manufacturer's instructions, mutagenesis was performed in two steps because of the primer length. For each mutant, two pairs of primers were designed ( Table 1) .
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