Author: Li, Lv; Dai, Hong-Juan; Ye, Mao; Wang, Shu-Ling; Xiao, Xiao-Juan; Zheng, Jie; Chen, Hui-Yong; Luo, Yu-hao; Liu, Jing
Title: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition Document date: 2012_11_23
ID: 13amcxh2_26
Snippet: Cell viability and cytotoxicity were measured with 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4disulfophenyl)-2H tetrazolium monosodium salt (CCK-8) assay as described previously. Briefly, exponentially growing K562 cells treated with various concentrations of lycorine (1.25, 2.5, or 5.0 μM) or without lycorine were cultivated at 1.25 × 10 4 cells/well in a 96-well tissue culture plate (BioCoat) at a total volume of 100 μL per well. Af.....
Document: Cell viability and cytotoxicity were measured with 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4disulfophenyl)-2H tetrazolium monosodium salt (CCK-8) assay as described previously. Briefly, exponentially growing K562 cells treated with various concentrations of lycorine (1.25, 2.5, or 5.0 μM) or without lycorine were cultivated at 1.25 × 10 4 cells/well in a 96-well tissue culture plate (BioCoat) at a total volume of 100 μL per well. After cells were incubated for 24 and 48 h, 10 μL of CCK-8 solution (Beyotime) was added to each well and incubation of cells was performed for another 4 h at 37°C. The relative cell viability was determined by scanning with an ELISA reader with a 450 nm filter and calculated by CCK-8 assay.
Search related documents:
Co phrase search for related documents- BioCoat culture plate and ELISA reader: 1
- BioCoat culture plate and μm lycorine: 1
- BioCoat culture plate and nm filter: 1
- BioCoat culture plate and tissue BioCoat culture plate: 1
- cell incubation and culture plate: 1
- cell incubation and ELISA reader: 1
- cell incubation and relative cell viability: 1, 2
- cell viability and culture plate: 1, 2, 3, 4, 5
- cell viability and cytotoxicity cell viability: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- cell viability and ELISA reader: 1, 2
- cell viability and relative cell viability: 1, 2, 3, 4, 5, 6, 7, 8, 9
- cell viability and total volume: 1
- culture plate and ELISA reader: 1, 2
- culture plate and μm lycorine: 1
- culture plate and nm filter: 1
- culture plate and tissue BioCoat culture plate: 1
- culture plate and total volume: 1, 2
- ELISA reader and μm lycorine: 1
- ELISA reader and nm filter: 1
Co phrase search for related documents, hyperlinks ordered by date