Selected article for: "initial viral load and virus detectable"
Author: Warner, Nikole L.; Jokinen, Jenny D.; Beier, Juliane I.; Sokoloski, Kevin J.; Lukashevich, Igor S.
Title: Mammarenaviral Infection Is Dependent on Directional Exposure to and Release from Polarized Intestinal Epithelia
  • Document date: 2018_2_10
    • ID: 1t8jmunt_22
    Snippet: In addition to the two strains of LCMV described above, the patterns of viral entry and release of ML-29 was assessed in the polarized Caco-2 model. As shown in ( Figure 4A ) Caco-2 cells apically infected with ML-29 failed to produce detectable virus particles from the basolateral surface, despite Supernatants were collected from both the insert, and well of the Transwells, to determine viral release from the apical or basolateral surfaces indep.....
    Document: In addition to the two strains of LCMV described above, the patterns of viral entry and release of ML-29 was assessed in the polarized Caco-2 model. As shown in ( Figure 4A ) Caco-2 cells apically infected with ML-29 failed to produce detectable virus particles from the basolateral surface, despite Supernatants were collected from both the insert, and well of the Transwells, to determine viral release from the apical or basolateral surfaces independent from one another. Viral titer was measured using standard plaque assay. Release from the Apical surface (black) and the Basolateral surface (red) is plotted with respect to time, with initial viral load subtracted. Values shown are the means of 3 biological replicates with the error bar representing the standard deviation of the mean. If viral plaque forming units (PFUs) were not observed, data received a place-holder value to signify samples were tested, but no data (ND) was collected. # indicates that one or more biological replicates was below limit of detection. * p-value ≤ 0.05.

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