Author: Zivcec, Marko; Scholte, Florine E. M.; Spiropoulou, Christina F.; Spengler, Jessica R.; Bergeron, Éric
Title: Molecular Insights into Crimean-Congo Hemorrhagic Fever Virus Document date: 2016_4_21
ID: 0a20s62q_39
Snippet: robust Renilla reporter activity that can be used to study cell entry [25] . In the second system, transcriptionally and entry competent VLPs (tec-VLPs) are generated using a codon-optimized version of the L protein and GPC, and a NanoLuc reporter to produce VLPs. The resulting tec-VLPs can enter target cells to generate a robust NanoLuc signal without the need to express L protein and NP in the target cells, simplifying workflow for studying ent.....
Document: robust Renilla reporter activity that can be used to study cell entry [25] . In the second system, transcriptionally and entry competent VLPs (tec-VLPs) are generated using a codon-optimized version of the L protein and GPC, and a NanoLuc reporter to produce VLPs. The resulting tec-VLPs can enter target cells to generate a robust NanoLuc signal without the need to express L protein and NP in the target cells, simplifying workflow for studying entry and primary transcription ( Figure 6 ) [27] .
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