Selected article for: "amplicon length and NCBI database"
Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
  • Document date: 2014_8_3
    • ID: 1e44usl6_12
    Snippet: We used the script simulate PCR.pl (https://sourceforge .net/projects/simulatepcr/ [7] ) to predict all PCR amplicons from the multiplex degenerate primers compared to the target sequences and to the NCBI nt database. This script is run automatically from the run tiled primers code after it predicts primers. It is set to predict amplicons up to twice the maximum amplicon length specified by the user......
    Document: We used the script simulate PCR.pl (https://sourceforge .net/projects/simulatepcr/ [7] ) to predict all PCR amplicons from the multiplex degenerate primers compared to the target sequences and to the NCBI nt database. This script is run automatically from the run tiled primers code after it predicts primers. It is set to predict amplicons up to twice the maximum amplicon length specified by the user.

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