Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes Document date: 2014_8_3
ID: 1e44usl6_15
Snippet: One amplicon per target sequence per region was desired (PriMux option file with -primer selection iterations = 1). Table 1 shows the average genome or segment length, the number of genomes available for each target, the % consensus among those sequences, and the total number of primers to amplify all overlapping regions of all genomes. All products from the nt database under 7800 bp (shorter amplicon) or 26 kb (longer amplicon) were predicted wi.....
Document: One amplicon per target sequence per region was desired (PriMux option file with -primer selection iterations = 1). Table 1 shows the average genome or segment length, the number of genomes available for each target, the % consensus among those sequences, and the total number of primers to amplify all overlapping regions of all genomes. All products from the nt database under 7800 bp (shorter amplicon) or 26 kb (longer amplicon) were predicted with simulate PCR to identify potential amplification of nontarget organisms (Tables 3 and 4 ).
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