Selected article for: "primer reverse and reverse primer"
Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
  • Document date: 2014_8_3
    • ID: 1e44usl6_23
    Snippet: The amplification of each primer pair in the multiplex was tested using a seminested PCR strategy to verify that the correct, specific amplicons were being produced from each multiplex of primers for a given region ( Figure 2 , Table S2 ). The multiplex PCR products served as templates for PCR reactions with primer pairs that included the reverse primer of one region paired with the forward primer from the downstream adjacent region to determine.....
    Document: The amplification of each primer pair in the multiplex was tested using a seminested PCR strategy to verify that the correct, specific amplicons were being produced from each multiplex of primers for a given region ( Figure 2 , Table S2 ). The multiplex PCR products served as templates for PCR reactions with primer pairs that included the reverse primer of one region paired with the forward primer from the downstream adjacent region to determine if the template generated from the multiplex was present. To ensure that the PCR product was generated from the multiplex product template rather than genomic DNA carried over from the initial sample, the multiplex product template was diluted 1 : 10,000 or excised from a gel and purified prior to use as a template.

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