Selected article for: "clinical sample and high throughput"
Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
  • Document date: 2014_8_3
    • ID: 1e44usl6_4
    Snippet: Possible applications include target enrichment for viral discovery of new members in a viral family from a complex host background, improving high throughput sequencing sensitivity and coverage of a rapidly evolving virus, or enriched coverage of variants in a gene family. We demonstrate the scalability of this software for designing whole genome amplification primers for a number of highly pathogenic viral groups which display very high levels .....
    Document: Possible applications include target enrichment for viral discovery of new members in a viral family from a complex host background, improving high throughput sequencing sensitivity and coverage of a rapidly evolving virus, or enriched coverage of variants in a gene family. We demonstrate the scalability of this software for designing whole genome amplification primers for a number of highly pathogenic viral groups which display very high levels of sequence variation, and for which we anticipate that targeted enrichment would be needed to obtain adequate sensitivity and genome coverage when sequencing from a clinical or environmental sample.

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