Author: Dyer, Wayne B; Zaunders, John J; Yuan, Fang Fang; Wang, Bin; Learmont, Jennifer C; Geczy, Andrew F; Saksena, Nitin K; McPhee, Dale A; Gorry, Paul R; Sullivan, John S
Title: Mechanisms of HIV non-progression; robust and sustained CD4+ T-cell proliferative responses to p24 antigen correlate with control of viraemia and lack of disease progression after long-term transfusion-acquired HIV-1 infection Document date: 2008_12_11
ID: 0ddutmdd_13
Snippet: Anti-HIV T cell function assays were performed as previously described [15, 29] . Briefly, the proliferative response to HIV-1 p24 was determined by 6 day culture of PBMC (1 × 10 5 cells/well) in RPMI medium with 15% pooled human serum in round bottom microtitre plates, with 2 μg/ml HIV-1 SF2 p24 (Chiron, Emeryville, CA, USA), or medium alone for control. After 6 days, proliferative responses were determined by 3 H-thymidine incorporation durin.....
Document: Anti-HIV T cell function assays were performed as previously described [15, 29] . Briefly, the proliferative response to HIV-1 p24 was determined by 6 day culture of PBMC (1 × 10 5 cells/well) in RPMI medium with 15% pooled human serum in round bottom microtitre plates, with 2 μg/ml HIV-1 SF2 p24 (Chiron, Emeryville, CA, USA), or medium alone for control. After 6 days, proliferative responses were determined by 3 H-thymidine incorporation during a further 6 hours culture, followed by cell harvest and reading in a liquid scintillation counter. Results were expressed as stimulation index (SI; mean counts antigen wells/mean counts control wells), and a SI >3 was considered a positive response.
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