Selected article for: "agarose gel and bp size"
Author: Barker, Emily N.; Stranieri, Angelica; Helps, Chris R.; Porter, Emily L.; Davidson, Andrew D.; Day, Michael J.; Knowles, Toby; Kipar, Anja; Tasker, Séverine
Title: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis
  • Document date: 2017_10_5
    • ID: 08b0g46x_22
    Snippet: Samples that failed to produce definitive pyrosequencing data were subjected to agarose gel (1% w/v) electrophoresis, using ethidium bromide stain, with EasyLadder I (50 ng/band; Bioline, London, UK) and analysed using a GelDoc-It ® Imaging System (UVP LLC, Cambridge, UK) to confirm that a single amplicon of the correct size (153 bp) had been produced using primers F614/ R766 (PCR as described above). Samples that produced a 153 bp amplicon were.....
    Document: Samples that failed to produce definitive pyrosequencing data were subjected to agarose gel (1% w/v) electrophoresis, using ethidium bromide stain, with EasyLadder I (50 ng/band; Bioline, London, UK) and analysed using a GelDoc-It ® Imaging System (UVP LLC, Cambridge, UK) to confirm that a single amplicon of the correct size (153 bp) had been produced using primers F614/ R766 (PCR as described above). Samples that produced a 153 bp amplicon were subjected to Sanger sequencing. Sequencing was performed using non-biotinylated amplification primers in a standard protocol (DNA Sequencing and Services, http://www.dnaseq.co.uk).

    Search related documents:
    Co phrase search for related documents
    • agarose gel and correct size: 1
    • agarose gel and Sanger sequencing: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • agarose gel and single amplicon: 1, 2
    • agarose gel and standard protocol: 1, 2
    • amplification primer and primer produce: 1
    • amplification primer and Sanger sequencing: 1, 2, 3, 4
    • amplification primer and single amplicon: 1
    • correct size and Sanger sequencing: 1
    • correct size and single amplicon: 1
    • correct size and standard protocol: 1
    • PCR describe and Sanger sequencing: 1, 2
    • Sanger sequencing and single amplicon: 1
    • Sanger sequencing and standard protocol: 1, 2, 3