Selected article for: "reagent Invitrogen and real time"
Author: Yang, Liu; Du, Xing; Liu, Lu; Cao, Qiuyu; Pan, Zengxiang; Li, Qifa
Title: miR-1306 Mediates the Feedback Regulation of the TGF-ß/SMAD Signaling Pathway in Granulosa Cells
  • Document date: 2019_3_31
    • ID: 16qix4ab_14
    Snippet: Total RNA was isolated from follicles and cultured GCs using TRIzol reagent (Invitrogen, USA), and then reverse-transcribed to cDNA using the PrimeScriptâ„¢ RT Master Mix (Perfect Real Time) (Takara, Beijing, China) according to the manufacturer's protocol. qRT-PCR was performed in triplicate with AceQ qPCR SYBR Green Master Mix (Takara, Beijing, China) using the ABI Step One system (Applied Biosystems, Foster City, CA, USA) according to the manu.....
    Document: Total RNA was isolated from follicles and cultured GCs using TRIzol reagent (Invitrogen, USA), and then reverse-transcribed to cDNA using the PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Beijing, China) according to the manufacturer's protocol. qRT-PCR was performed in triplicate with AceQ qPCR SYBR Green Master Mix (Takara, Beijing, China) using the ABI Step One system (Applied Biosystems, Foster City, CA, USA) according to the manufacture's instruction. The mRNA levels of TGFBR2 and DiGeorge Syndrome Chromosomal Region 8 (DGCR8) were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and U6 small nuclear RNA (U6) was used as the internal control for miRNA. The 2 −∆∆CT method was used to normalize the relative levels of the target genes. The details of all the primers for qRT-PCR used in this study are provided in Supplementary Table S2 .

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