Author: Takhampunya, Ratree; Korkusol, Achareeya; Pongpichit, Chalermpol; Yodin, Komsan; Rungrojn, Artharee; Chanarat, Nitima; Promsathaporn, Sommai; Monkanna, Taweesak; Thaloengsok, Sasikanya; Tippayachai, Bousaraporn; Kumfao, Naruemon; Richards, Allen L.; Davidson, Silas A.
Title: Metagenomic Approach to Characterizing Disease Epidemiology in a Disease-Endemic Environment in Northern Thailand Document date: 2019_2_26
ID: 0gi6qzw0_16
Snippet: Spleen and kidney tissues from each rodent were cut into pieces (∼3 mm in diameter) and added to 230 µl of ATL Tissue Lysis Buffer and 20 µl of Proteinase K solution (20 mg/ml), then incubated at 55 • C for 1 h or until the tissues were homogenized. A total volume of 250 µl homogenized solution was then used for DNA extraction on the QIAsymphony R SP with QIAsymphony R DNA Mini Kit and Tissue HC 200 DSP protocol. The DNA was eluted in 200 .....
Document: Spleen and kidney tissues from each rodent were cut into pieces (∼3 mm in diameter) and added to 230 µl of ATL Tissue Lysis Buffer and 20 µl of Proteinase K solution (20 mg/ml), then incubated at 55 • C for 1 h or until the tissues were homogenized. A total volume of 250 µl homogenized solution was then used for DNA extraction on the QIAsymphony R SP with QIAsymphony R DNA Mini Kit and Tissue HC 200 DSP protocol. The DNA was eluted in 200 µl and stored at −20 • C until use. Ultrapure DNA/RNA-free distilled water was also included as an extraction control.
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