Author: Takhampunya, Ratree; Korkusol, Achareeya; Pongpichit, Chalermpol; Yodin, Komsan; Rungrojn, Artharee; Chanarat, Nitima; Promsathaporn, Sommai; Monkanna, Taweesak; Thaloengsok, Sasikanya; Tippayachai, Bousaraporn; Kumfao, Naruemon; Richards, Allen L.; Davidson, Silas A.
Title: Metagenomic Approach to Characterizing Disease Epidemiology in a Disease-Endemic Environment in Northern Thailand Document date: 2019_2_26
ID: 0gi6qzw0_25
Snippet: The final products were cleaned using Agencourt AMPure XP beads. The purity of the libraries was checked on the QIAxcel Advanced System (Qiagen) with a QIAxcel DNA High Resolution Cartridge. Purified amplicon libraries were quantified using the Qubit dsDNA HS Assay Kit (Invitrogen). DNA concentration was calculated and normalized to reach 4.0 nM for each library. Five microliters of DNA from each library were pooled (each NGS pool had 29-78 DNA l.....
Document: The final products were cleaned using Agencourt AMPure XP beads. The purity of the libraries was checked on the QIAxcel Advanced System (Qiagen) with a QIAxcel DNA High Resolution Cartridge. Purified amplicon libraries were quantified using the Qubit dsDNA HS Assay Kit (Invitrogen). DNA concentration was calculated and normalized to reach 4.0 nM for each library. Five microliters of DNA from each library were pooled (each NGS pool had 29-78 DNA libraries) for a NGS run (1-5 runs in total). Pooled libraries were denatured and diluted to a final concentration of 8 pM with a 10% PhiX (Illumina) control. Sequencing was performed using the MiSeq Reagent Kit V3 on the Illumina MiSeq System.
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