Selected article for: "high throughput and HTS high throughput sequencing"
Author: Chen, Qi; Wang, Leyi; Zheng, Ying; Zhang, Jianqiang; Guo, Baoqing; Yoon, Kyoung-Jin; Gauger, Phillip C.; Harmon, Karen M.; Main, Rodger G.; Li, Ganwu
Title: Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States
  • Document date: 2018_5_25
    • ID: 1nyhllij_3
    Snippet: Using virus-specific PCRs, viruses such as PDCoV and PRoV have been detected along with PEDV in some diarrheic cases submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISUVDL) and other diagnostic laboratories of different states [7, 8] . However, there have been no reports thoroughly investigating viruses concurrent co-infections with PEDV in diarrheic pigs. The random primers-based, hypothesis-free High-throughput sequenci.....
    Document: Using virus-specific PCRs, viruses such as PDCoV and PRoV have been detected along with PEDV in some diarrheic cases submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISUVDL) and other diagnostic laboratories of different states [7, 8] . However, there have been no reports thoroughly investigating viruses concurrent co-infections with PEDV in diarrheic pigs. The random primers-based, hypothesis-free High-throughput sequencing (HTS) enables simultaneous detection of multiple microorganisms, making it possible to identify not only known pathogens, but also novel and/ or uncharacterized potential pathogens. In this study, a HTS-based metagenomics approach was used to characterize the fecal RNA virome in diarrheic pigs infected with PEDV. Some previously uncharacterized viruses were detected and genetically characterized in the present study.

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