Selected article for: "DNeasy kit and Germany Hilden Qiagen DNeasy kit"
Author: Allemandou, Aude; Grasland, Béatrice; Hernandez-Nignol, Anne-Cécile; Kéranflec'h, André; Cariolet, Roland; Jestin, André
Title: Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein
  • Document date: 2011_3_24
    • ID: 1fl0we2a_29
    Snippet: Two hundred milligrams of each tissue sample were homogenized in phosphate buffer saline (PBS) using the mixer mill MM301 (Retsch, Haan, Germany). Virus DNA was extracted from 200 μL of serum with the Wizard SV96 Genomic DNA Purification system (Promega, Madison, WI, USA) or from 80 μL of tissue homogenate with the DNeasy tissue kit (Qiagen, Hilden, Germany) according to the manufacturers' recommendations. Following the DNA extraction step, PCV.....
    Document: Two hundred milligrams of each tissue sample were homogenized in phosphate buffer saline (PBS) using the mixer mill MM301 (Retsch, Haan, Germany). Virus DNA was extracted from 200 μL of serum with the Wizard SV96 Genomic DNA Purification system (Promega, Madison, WI, USA) or from 80 μL of tissue homogenate with the DNeasy tissue kit (Qiagen, Hilden, Germany) according to the manufacturers' recommendations. Following the DNA extraction step, PCV-2 genomes were quantified by a previously described PCV-2 TaqMan real-time PCR assay based on the amplification of a PCV-2a and PCV-2b genomic target in the capsid gene at 54 and 60°C [21] .

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