Author: Li, Zi; Lan, Yungang; Zhao, Kui; Lv, Xiaoling; Ding, Ning; Lu, Huijun; Zhang, Jing; Yue, Huiqing; Shi, Junchao; Song, Deguang; Gao, Feng; He, Wenqi
Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1 Document date: 2017_5_3
ID: 07b3pbxc_17
Snippet: For immunofluorescence staining, fresh brain tissues frozen in liquid nitrogen and embedded in OCT compound were cut into cryostat sections (8 µm) and mounted on Superfrost Plus slides. Before staining, the slides were warmed at room temperature for 30 min and were then immersed in blocking buffer (10% serum and 0.1% Triton X-100 in PBS) for 1 h at room temperature. Slides were then treated with primary antibodies overnight at 4 • C and subseq.....
Document: For immunofluorescence staining, fresh brain tissues frozen in liquid nitrogen and embedded in OCT compound were cut into cryostat sections (8 µm) and mounted on Superfrost Plus slides. Before staining, the slides were warmed at room temperature for 30 min and were then immersed in blocking buffer (10% serum and 0.1% Triton X-100 in PBS) for 1 h at room temperature. Slides were then treated with primary antibodies overnight at 4 • C and subsequently incubated with secondary antibodies conjugated with either Alexa 488 or Alexa 594 for 30 min at room temperature. After counterstaining the samples, the coverslips were mounted and viewed under a confocal microscope.
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