Author: Adedeji, Adeyemi O.; Marchand, Bruno; te Velthuis, Aartjan J. W.; Snijder, Eric J.; Weiss, Susan; Eoff, Robert L.; Singh, Kamalendra; Sarafianos, Stefan G.
Title: Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase Document date: 2012_5_15
ID: 1ssh296a_12
Snippet: To investigate whether nucleic acid unwinding depends on the loading strand length and to determine the minimum length of 59single-stranded (ss) overhang required for efficient helicase activity we prepared 4 partial duplex DNA substrates ( Figure S1 and Figure 3C ) with varying lengths of 59-end ssDNAs (between 5-to 20-bases), but contained same length of dsDNA duplex region (23/18-mer, 28/18-mer, 31/18-mer, and 38/18-mer). Using these substrate.....
Document: To investigate whether nucleic acid unwinding depends on the loading strand length and to determine the minimum length of 59single-stranded (ss) overhang required for efficient helicase activity we prepared 4 partial duplex DNA substrates ( Figure S1 and Figure 3C ) with varying lengths of 59-end ssDNAs (between 5-to 20-bases), but contained same length of dsDNA duplex region (23/18-mer, 28/18-mer, 31/18-mer, and 38/18-mer). Using these substrates we monitored the unwinding activity of GST-nsp13. GST-nsp13 could not unwind the 23/18-mer that has only 5 bases ssDNA at the 59-end, but it did unwind the 28/18-mer (10 bases ssDNA at 59-end), 31/18-mer (13 bases ssDNA at 59-end), and 38/ 18-mer (20 bases at 59-end), suggesting that it requires more than 5-base-long 59-loading strand ( Figure 3C ).
Search related documents:
Co phrase search for related documents- dna substrate and duplex region: 1
- dna substrate and helicase activity: 1, 2, 3, 4, 5, 6, 7, 8
- dna substrate and loading strand length: 1
- dna substrate and nucleic acid unwinding: 1
- dna substrate and unwinding activity: 1, 2, 3, 4, 5
- duplex dna substrate and helicase activity: 1, 2
- duplex dna substrate and nucleic acid unwinding: 1
- duplex dna substrate and unwinding activity: 1
- duplex region and helicase activity: 1, 2
- duplex region and nucleic acid unwinding: 1
- duplex region and unwinding activity: 1
- efficient helicase activity and helicase activity: 1
- helicase activity and loading strand length: 1
- helicase activity and minimum length: 1
- helicase activity and nucleic acid unwinding: 1, 2, 3, 4, 5, 6, 7, 8
- helicase activity and unwinding activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- loading strand length and minimum length: 1
- loading strand length and nucleic acid unwinding: 1
- minimum length and nucleic acid unwinding: 1
Co phrase search for related documents, hyperlinks ordered by date