Author: Adedeji, Adeyemi O.; Marchand, Bruno; te Velthuis, Aartjan J. W.; Snijder, Eric J.; Weiss, Susan; Eoff, Robert L.; Singh, Kamalendra; Sarafianos, Stefan G.
Title: Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase Document date: 2012_5_15
ID: 1ssh296a_22
Snippet: Sequence alignment of nsp13 with other helicases suggests that the SARS-CoV nsp13 belongs to the SF1 family of helicases [34] . However, several biochemical aspects of the enzymatic mechanism of SARS-CoV nsp13 are still unknown, including ATP hydrolysis, translocation along the nucleic acid, and the unwinding rate. The present study fills this knowledge gap and establishes the minimal kinetic mechanism of the enzyme. Moreover, it provides the fir.....
Document: Sequence alignment of nsp13 with other helicases suggests that the SARS-CoV nsp13 belongs to the SF1 family of helicases [34] . However, several biochemical aspects of the enzymatic mechanism of SARS-CoV nsp13 are still unknown, including ATP hydrolysis, translocation along the nucleic acid, and the unwinding rate. The present study fills this knowledge gap and establishes the minimal kinetic mechanism of the enzyme. Moreover, it provides the first insights into how interactions between the helicase and polymerase enzymes enhance the efficiency of replication in coronaviruses.
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