Author: Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.
Title: NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes Document date: 2016_5_4
ID: 0ozzbp85_13
Snippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro cDNA synthesis kit, both according to the manufacturer's instructions. The cDNA was then quantitated by PCR using Maxima SYBR Green master mix (Thermo) and a StepOnePlus™ Real Time PCR system (Applied Biosystems). Primers were obtained from Sigma and are listed in S1 Table. The 2 -ΔΔCt m.....
Document: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro cDNA synthesis kit, both according to the manufacturer's instructions. The cDNA was then quantitated by PCR using Maxima SYBR Green master mix (Thermo) and a StepOnePlus™ Real Time PCR system (Applied Biosystems). Primers were obtained from Sigma and are listed in S1 Table. The 2 -ΔΔCt method was used to determine relative transcript levels using the housekeeping GAPDH gene to normalize the data [45] .
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